Effect of H- and O-termination of nanocrystalline diamond films on cell adhesion and osteogenic differentiation

0440278 - FZÚ 2015 RIV CZ eng C - Conference Paper (international conference)
Lišková, Jana - Babchenko, Oleg - Varga, Marián - Kromka, Alexander - Hadraba, Daniel - Švindrych, Zdeněk - Bačáková, Lucie
Effect of H- and O-termination of nanocrystalline diamond films on cell adhesion and osteogenic differentiation.
NANOCON 2014 Conference Proceedings. Ostrava: Tanger, 2014. ISBN 978-80-87294-55-0.
[International Conference NANOCON /6./. Brno (CZ), 05.11.2014-07.11.2014]
R&D Projects: GA ČR(CZ) GA14-04790S; GA MŠMT(CZ) EE2.3.30.0025; GA MŠMT(CZ) ED1.1.00/02.0109
Grant - others:MSMT(CZ) CZ.1.05/1.1.00/02.0109
Institutional support: RVO:68378271 ; RVO:67985823
Keywords : nanocrystalline diamond film * osteoblast * MSC * type I collagen * paxillin
Subject RIV: BM - Solid Matter Physics ; Magnetism

In order to control the hydrophobic or hydrophilic character, the NCD film surfaces were grafted by hydrogen atoms (H-termination) or by oxygen atoms (O-termination). Human mesenchymal stem cells and primary human osteoblasts were used for biological studies on H- and O-terminated NCD films. We found that cells cultivated on O-terminated NCD films exhibit better adhesion than H-terminated NCD films. In addition, the expression of osteogenic cell markers collagen and alkaline phosphatase analysed by Realtime PCR and immunostaining was higher on O-terminated films.
Permanent Link: http://hdl.handle.net/11104/0243399