Plant Cell Morphogenesis: Methods and Protocols

0439872 - FGÚ 2015 RIV US eng M - Monography Chapter
Albrechtová, J. - Kubínová, Z. - Soukup, A. - Janáček, Jiří
Image Analysis: Basic Procedures for Description of Plant Structures.
Plant Cell Morphogenesis: Methods and Protocols. Vol. 1080. New York: Humana Press, 2014 - (Žárský, V.; Cvrčková, F.), s. 67-76. Methods in Molecular Biology. ISBN 978-1-62703-643-6
R&D Projects: GA ČR(CZ) GAP501/10/0340
Institutional support: RVO:67985823
Keywords : experiment design * image analysis * stereology
Subject RIV: EA - Cell Biology

This chapter gives examples of basic procedures of quantification of plant structures with the use of image analysis, which are commonly employed to describe differences among experimental treatments or phenotypes of plant material. Tasks are demonstrated with the use of ImageJ, a widely used public domain Java image processing program. Principles of sampling design based on systematic uniform random sampling for quantitative studies of anatomical parameters are given to obtain their unbiased estimations and simplified "rules of thumb" are presented. The basic procedures mentioned in the text are (1) sampling, (2) calibration, (3) manual length measurement, (4) leaf surface area measurement, (5) estimation of particle density demonstrated on an example of stomatal density, and (6) analysis of epidermal cell shape
Permanent Link: http://hdl.handle.net/11104/0243056