Diversity and transmission of soil transmitted strongylid nematodes between humans and other primates in the wild

0439385 - ÚBO 2015 US eng A - Abstract
Kalousová, B. - Hasegawa, H. - Modrý, D. - Kitagawa, M. - Shutt, K. A. - Todd, A. - Profousová, I. - Petrželková, Klára Judita
Diversity and transmission of soil transmitted strongylid nematodes between humans and other primates in the wild.
Journal of Nematology. Roč. 46, č. 2 (2014), s. 182. ISSN 0022-300X
Institutional support: RVO:68081766
Subject RIV: EG - Zoology

Humans have historically shared habitat with nonhuman primates. However, the dynamics of human-primate interactions have recently changed dramatically and increased as a result of forest fragmentation, habitat encroachment and tourism. Little is known about the diversity and transmission of helminthes between humans and other primates and the presence of potential zoonotic strongylids should be viewed as a major concern for both public health and primate conservation. Generic diagnosis of strongylids is traditionally facilitated by morphological examination of L3 larvae; however, coprocultures for the development of larvae are not commonly performed in routine diagnostics of strongylids in primates. The difficulties in diagnosing hookworms combined with their clinical relevance have driven the need for molecular research/diagnostic approaches. We therefore studied the diversity and transmission of soil transmitted strongylid nematodes between humans and other primates inhabiting Dzanga Sangha Protected Areas, Central African Republic and Campo Ma’an National Park, Cameroon. We developed the filariform larvae of hookworms from faeces by using Harada-Mori filter-paper culture and identified them based on morphology. We sequenced and compared the first and second internal transcribed spacers (ITS-1 and ITS-2) of nuclear ribosomal DNA and partial cytochrome c oxidase subunit 1 (cox1) gene of mtDNA obtained from larvae to identify the molecular type. Our data revealed an as yet undocumented diversity of hookworms (Ancylostomatoidea: Ancylostomatidae) in western lowland gorillas, chimpanzees, agile mangabeys and humans and provided evidence for the transmission of some parasite taxa among primates and humans as well as the discovery of a novel nematode species affecting humans.
Permanent Link: http://hdl.handle.net/11104/0242676