Optical Biosensor of Biogenic Amines with Immobilized Enzyme

0439175 - ÚCHP 2015 GB eng C - Conference Paper (international conference)
Maixnerová, Lucie - Horvitz, Alexandar - Kuncová, Gabriela - Přibyl, M. - Šebela, M. - Koštejn, Martin
Optical Biosensor of Biogenic Amines with Immobilized Enzyme.
Innovations in Encapsulation 2014. -: -, 2014, s. 36. ISBN N.
[Innovations in Encapsulation 2014. London (GB), 12.12.2014]
R&D Projects: GA TA ČR TA03010544
Institutional support: RVO:67985858
Keywords : optical enzymatic biosensor * biogenic amines * mathematical model
Subject RIV: CB - Analytical Chemistry, Separation

We studied optical biosensor for BA with an optical oxygen transducer that contained an enzyme diamine oxidase (DAO) from pea and ruthenium complex. Both components were incorporated in chemically stable inorganic-organic polymer Ormocer® to form sensitive layers. The principle of the measurement with this biosensor lies in oxygen consumption due to oxidation of BA catalyzed by DAO. Ruthenium complex serves as an optical transducer.
Permanent Link: http://hdl.handle.net/11104/0242468