Reproducible and time-course study of yeast biofilm by Raman spectroscopy

0437746 - ÚPT 2015 RIV US eng C - Conference Paper (international conference)
Samek, Ota - Mlynariková, K. - Bernatová, Silvie - Ježek, Jan - Krzyžánek, Vladislav - Šiler, Martin - Zemánek, Pavel - Růžička, F.
Reproducible and time-course study of yeast biofilm by Raman spectroscopy.
19th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics (Proceedings of SPIE 9441). Bellingham: SPIE, 2014, 94411M:1-9. ISBN 9781628415568. ISSN 0277-786X.
[Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics /19./. Jelenia Góra (PL), 08.09.2014-12.09.2014]
R&D Projects: GA ČR GAP205/11/1687; GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institutional support: RVO:68081731
Keywords : Raman spectroscopy * yeast * chemometrics * diagnostics * principal component analysis
Subject RIV: BH - Optics, Masers, Lasers

We report on Raman spectroscopy measurements - separated by a given time intervals - for the selected yeast strains (biofilm positive and biofilm negative) on colonies grown directly on the Petri dishes or on the well-plate. Chemometric principal component analysis of these spectra sets generated clusters of data points, from which the reproducibility of the measurement could be analysed. Consequently, these resulted in clusters coinciding well with the biofilm positive and biofilm negative strains measurement of a particular sample dish, suggesting good reproducibility of our measurement procedure, even when the samples were prepared and measured days up to months apart. This suggests the potential of Raman spectroscopy in routine clinical diagnostic.
Permanent Link: http://hdl.handle.net/11104/0241267