Capillary Electrophoresis of Carbohydrates: From Monosaccharides to Complex Plysaccharides

0437343 - ÚIACH 2015 RIV US eng M - Monography Chapter
Růžička, F. - Horká, Marie - Holá, V.
Extracellular Polysaccharides in Microbial Biofilm and Their Influence on the Electrophoretic Properties of Microbial Cells.
Capillary Electrophoresis of Carbohydrates: From Monosaccharides to Complex Plysaccharides. Totowa: Humana Press Inc, 2011 - (Volpi, N.), s. 105-126. ISBN 978-1-60761-874-4
Institutional support: RVO:68081715
Keywords : capillary zone electrophoresis * laser induced flurescence * high performance electrophoresis
Subject RIV: CB - Analytical Chemistry, Separation

The surfaces of biofilm-positive microorganisms are usually covered with biofilm-specific extracellular polysaccharide substances that play a key role in a biofilm formation and function [1,2] The presence of this substance on the surface can affect the physicochemical properties of the bacterial cell, including the cell-surface hydrophobicity and surface charge The differences in the surface charges lead to the different isoelectric points and the different electromigration characteristics of biofilm-positive and biofilm-negative cells For this reason, the use of the capillary electrophoresis, especially capillary isoelectric focusing (CIEF) and capillary zone electrophoresis (CZE) [3,4], appears to be the appropriate method for the detection of biofilm positivity in a particular strain, as well as for the separation of biofilm-positive and biofilm-negative strains.
Permanent Link: http://hdl.handle.net/11104/0240944