Clustered DNA damage on subcellular level: effect of scavengers

0436873 - ÚJF 2015 RIV US eng J - Journal Article
Pachnerová Brabcová, Kateřina - Sihver, L. - Yasuda, N. - Matuo, Y. - Štěpán, Václav - Davídková, Marie
Clustered DNA damage on subcellular level: effect of scavengers.
Radiation and Environmental Biophysics. Roč. 53, č. 4 (2014), s. 705-712. ISSN 0301-634X. E-ISSN 1432-2099
R&D Projects: GA MŠMT LD12008
Institutional support: RVO:61389005
Keywords : clustered damage * indirect effects * haevy ion * plasmid in liquid water * scavenger
Subject RIV: BO - Biophysics
Impact factor: 1.528, year: 2014

Clustered DNA damages are induced by ionizing radiation, particularly of high linear energy transfer (LET). Compared to isolated DNA damage sites, their biological effects can be more severe. We investigated a clustered DNA damage induced by high LET radiation (C 290 MeV u(-1) and Fe 500 MeV u(-1)) in pBR322 plasmid DNA. The plasmid is dissolved in pure water or in aqueous solution of one of the three scavengers (coumarin-3-carboxylic acid, dimethylsulfoxide, and glycylglycine). The yield of double strand breaks (DSB) induced in the DNA plasmid-scavenger system by heavy ion radiation was found to decrease with increasing scavenging capacity due to reaction with hydroxyl radical, linearly with high correlation coefficients. The yield of non-DSB clusters was found to occur twice as much as the DSB. Their decrease with increasing scavenging capacity had lower linear correlation coefficients. This indicates that the yield of non-DSB clusters depends on more factors, which are likely connected to the chemical properties of individual scavengers.
Permanent Link: http://hdl.handle.net/11104/0240517