Raman tweezers on bacteria: following the mechanisms of bacteriostatic versus bactericidal action

0436841 - ÚPT 2015 RIV US eng C - Conference Paper (international conference)
Bernatová, Silvie - Samek, Ota - Pilát, Zdeněk - Šerý, Mojmír - Ježek, Jan - Jákl, Petr - Šiler, Martin - Krzyžánek, Vladislav - Zemánek, Pavel - Holá, V. - Dvořáčková, M. - Růžička, F.
Raman tweezers on bacteria: following the mechanisms of bacteriostatic versus bactericidal action.
Biophotonics: Photonic Solutions for Better Health Care IV (Proceedings of SPIE 9129). Bellingham: SPIE, 2014, 91291Y:1-7. ISBN 9781628410778. ISSN 0277-786X.
[Biophotonics: Photonic Solutions for Better Health Care /4./. Brussels (BE), 14.04.2014-17.04.2014]
R&D Projects: GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01; GA ČR GAP205/11/1687
Institutional support: RVO:68081731
Keywords : bacteria * microorganisms * principal component analysisp * Raman scattering
Subject RIV: BH - Optics, Masers, Lasers

tweezers represents a unique method for identification of different microorganisms on the basis of Raman scattering. Raman tweezers allows us to fix and sterile manipulate with the trapped object and in the same time check the growth, viability, response to the external environment etc. by Raman signal evaluating. The investigations presented here include distinction of bacteria in general (staphylococcal cells), identification of bacteria strains (biofilm-positive and biofilm-negative) by using principal component analysis (PCA) and monitoring the influence of antibiotics.
Permanent Link: http://hdl.handle.net/11104/0240487