0436781 - ÚMCH 2015 RIV CH eng J - Journal Article
Kučerová, J. - Svobodová, Z. - Knotek, P. - Palarčik, J. - Vlček, Milan - Kincl, Miloslav - Horák, Daniel - Autebert, J. - Viovy, J.-L. - Bílková, Z.
PEGylation of magnetic poly(glycidyl methacrylate) microparticles for microfluidic bioassays.
Materials Science & Engineering C-Materials for Biological Applications. Roč. 40, 1 July (2014), s. 308-315. ISSN 0928-4931. E-ISSN 1873-0191
R&D Projects: GA MŠMT 7E12053; GA MŠMT 7E09109
EU Projects: European Commission(XE) 246513 - NADINE; European Commission(XE) 228980 - CAMINEMS
Institutional support: RVO:61389013
Keywords : PEGylation * magnetic microscopy * microfluidics
Subject RIV: CE - Biochemistry
Impact factor: 3.088, year: 2014 ; AIS: 0.624, rok: 2014
DOI: https://doi.org/10.1016/j.msec.2014.04.011

Magnetic poly(glycidyl methacrylate) microparticles containing carboxyl groups (PGMA-COOH) were coated using highly hydrophilic polymer poly(ethylene glycol) (PEG). PEG was used to reduce nonspecific interactions with proteins and cells. Zeta potential measurement, infrared spectroscopy, scanning electron microscopy, anti-PEG ELISA assay, and bioaffinity interactions between biotin and streptavidin-HRP successfully proved the presence of PEG on the surface of microspheres.
Permanent Link: http://hdl.handle.net/11104/0240475