0436621 - ÚPT 2015 RIV US eng J - Journal Article
Krzyžánek, Vladislav - Hrubanová, Kamila - Nebesářová, J. - Růžička, F.
Cryo-SEM of Perpendicular Cross Freeze-Fractures Through a High-Pressure-frozen Biofilm.
Microscopy and Microanalysis. Roč. 20, S3 (2014), s. 1232-1233. ISSN 1431-9276. E-ISSN 1435-8115
R&D Projects: GA MŠMT EE.2.3.20.0103; GA MŠMT(CZ) LO1212; GA TA ČR TE01020118; GA ČR GAP205/11/1687
Institutional support: RVO:68081731
Keywords : biofilm * cryo-SEM * high-pressure-freezing
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering
Impact factor: 1.872, year: 2014

The cryo scanning electron microscopy (cryo-SEM) as well as high-pressure-freezing (HPF) belongs to cutting edge techniques in electron microscopy of hydrated samples. However, their combination is not always easily applicable. Here, we present a way of combining high-pressure-freezing using EM PACT2 (Leica Microsystems) that fixes hydrated samples on a 1.4 mm sapphire discs and high resolution SEM JEOL 7401F equipped with the cryo-attachment ALTO 2500 (GATAN). The freeze-fracturing technique is used here for investigation of yeast cultures cultivated on the sapphire discs, where we focus on the formation of the extracellular matrix produced during the cultivation.
Permanent Link: http://hdl.handle.net/11104/0240330