Instability of cerebrospinal fluid after delayed storage and repeated freezing: a holistic study by drop coating deposition Raman spectroscopy

0436423 - MBÚ 2015 RIV DE eng J - Journal Article
Klener, J. - Hofbauerová, Kateřina - Bartoš, A. - Říčný, J. - Řípová, D. - Kopecký, V. Jr.
Instability of cerebrospinal fluid after delayed storage and repeated freezing: a holistic study by drop coating deposition Raman spectroscopy.
Clinical Chemistry and Laboratory Medicine. Roč. 52, č. 5 (2014), s. 657-664. ISSN 1434-6621. E-ISSN 1437-4331
Institutional support: RVO:61388971
Keywords : Alzheimer's disease * cerebrospinal fluid * cold denaturation
Subject RIV: EC - Immunology
Impact factor: 2.707, year: 2014

The handling of cerebrospinal fluid (CSF) affects the biomarker quantification used to diagnose Alzheimer's disease (AD). Only specialized centers can test for AD markers. The precise timing and freezing is required to correctly measure these biomarkers. Therefore, the effects of CSF storage temperature and repeated freeze/thaw cycles on CSF stability were investigated. Drop coating deposition Raman spectroscopy in combination with principal component analysis was used to analyze CSF and its dialyzed form (ELISA confirmed the removal of up to 80% of the AD markers). The advantage of this approach is that no prior knowledge of the biomarkers is necessary and that both the concentration and the protein structure of intact CSF are analyzed.
Permanent Link: http://hdl.handle.net/11104/0240276