The Study of Naphthoquinones and Their Complexes with DNA by Using Raman Spectroscopy and Surface Enhanced Raman Spectroscopy: New Insight into Interactions of DNA with Plant Secondary Metabolites

0435977 - BFÚ 2015 RIV US eng J - Journal Article
Vaverková, V. - Vrána, Oldřich - Adam, V. - Pekárek, T. - Jampílek, J. - Babula, P.
The Study of Naphthoquinones and Their Complexes with DNA by Using Raman Spectroscopy and Surface Enhanced Raman Spectroscopy: New Insight into Interactions of DNA with Plant Secondary Metabolites.
BioMed Research International. Roč. 2014, June (2014). ISSN 2314-6133. E-ISSN 2314-6141
Institutional support: RVO:68081707
Keywords : CALF-THYMUS DNA * FLUORESCENT SYMMETRIC PHENAZINES * VITAMIN-K CYCLE
Subject RIV: BO - Biophysics
Impact factor: 1.579, year: 2014

Naphthoquinones represent the group of plant secondary metabolites with cytotoxic properties based on their ability to generate reactive oxygen species and interfere with the processes of cell respiration. Due to this fact, the possible cytotoxic mechanisms on cellular and subcellular levels are investigated intensively. There are many targets of cytotoxic action on the cellular level; however, DNA is a critical target of many cytotoxic compounds. Due to the cytotoxic properties of naphthoquinones, it is necessary to study the processes of naphthoquinones, DNA interactions (1,4-naphthoquinone, binapthoquinone, juglone, lawsone, plumbagin), especially by using modern analytical techniques. In our work, the Raman spectroscopy was used to determine the possible binding sites of the naphthoquinones on the DNA and to characterize the bond of naphthoquinone to DNA. Experimental data reveals the relationships between the perturbations of structure-sensitive Raman bands and the types of the naphthoquinones involved.
Permanent Link: http://hdl.handle.net/11104/0239821