0435966 - BFÚ 2015 RIV CZ eng J - Journal Article
Stixová, Lenka - Hrušková, Tereza - Sehnalová, Petra - Legartová, Soňa - Svidenská, S. - Kozubek, Stanislav - Bártová, Eva
Advanced Microscopy Techniques Used for Comparison of UVA- and gamma-Irradiation-Induced DNA Damage in the Cell Nucleus and Nucleolus.
Folia Biologica. Roč. 60, č. 1 (2014), s. 76-84. ISSN 0015-5500. E-ISSN 0015-5500
R&D Projects: GA ČR GBP302/12/G157; GA MŠMT(CZ) EE2.3.30.0030
Institutional support: RVO:68081707
Keywords : UVA * gamma-irradiation * DNA damage response
Subject RIV: BO - Biophysics
Impact factor: 1.000, year: 2014

Every day, genomes are affected by genotoxic factors that create multiple DNA lesions. Several DNA repair systems have evolved to counteract the deleterious effects of DNA damage. These systems include a set of DNA repair mechanisms, damage tolerance processes, and activation of cell-cycle checkpoints. This study describes selected confocal microscopy techniques that investigate DNA damage-related nuclear events after UVA- and gamma-irradiation and compare the DNA damage response (DDR) induced by the two experimental approaches. In both cases, we observed induction of the nucleotide excision repair (NER) pathway and formation of localized double-strand breaks (DSBs). This was confirmed by analysis of cyclobutane pyrimidine dimers (CPDs) in the DNA lesions and by increased levels of gamma H2AX and 53BP1 proteins in the irradiated genome. DNA damage by UVA-lasers was potentiated by either BrdU or Hoechst 33342 pre-sensitization and compared to non-photosensitized cells.
Permanent Link: http://hdl.handle.net/11104/0239812