0435787 - MBÚ 2015 RIV NL eng J - Journal Article
Krásný, Lukáš - Rohlová, E. - Růžičková, E. - Šantrůček, J. - Hynek, R. - Hochel, I.
Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis.
Journal of Microbiological Methods. Roč. 98, MAR 2014 (2014), s. 105-113. ISSN 0167-7012. E-ISSN 1872-8359
R&D Projects: GA ČR GAP503/10/0664
Institutional support: RVO:61388971
Keywords : Biotyper * Cronobacter * MALDI-TOF
Subject RIV: CE - Biochemistry
Impact factor: 2.026, year: 2014 ; AIS: 0.672, rok: 2014
DOI: https://doi.org/10.1016/j.mimet.2014.01.008

Intact cell MALDI-TOF mass spectrometry is a rapid tool for the identification and classification of microorganisms, now widely used even in clinical laboratories. However, its distinctive power is not sufficient for some closely-related species. The genus Cronobacter, formerly known as Enterobacter sakazakii, contains such species. In this work, a new method for the differentiation of five Cronobacter species is presented involving the tryptic digestion of cytoplasmatic proteins followed by MALDI mass spectrometry analysis. A database was developed for use in Bruker Biotyper software including 52 reference spectra and tested on a set of 45 samples with an overall accuracy of about 80%. The possibility of measurement automation and the short time and low cost requirements of this method compared to those of biochemical tests or PCR methods make it a supplementary option to intact cell MALDI, providing additional information about the differentiation of problematic species
Permanent Link: http://hdl.handle.net/11104/0239660