Phthalates Deregulate Cell Proliferation, but Not Neuroendocrine Transdifferentiation, in Human LNCaP Prostate Cancer Cell Model

0435171 - BFÚ 2015 RIV CZ eng J - Journal Article
Hrubá, Eva - Pernicová, Zuzana - Palková, L. - Souček, Karel - Vondráček, Jan - Machala, M.
Phthalates Deregulate Cell Proliferation, but Not Neuroendocrine Transdifferentiation, in Human LNCaP Prostate Cancer Cell Model.
Folia Biologica. Roč. 60, č. 2014 (2014), s. 56-61. ISSN 0015-5500. E-ISSN 0015-5500
R&D Projects: GA MŠMT(CZ) EE2.3.30.0030; GA MŠMT(CZ) ED1.100/02/0123
Institutional support: RVO:68081707
Keywords : phthalates * prostate cancer cells * cell cycle modulation
Subject RIV: BO - Biophysics
Impact factor: 1.000, year: 2014

Phthalate esters are ubiquitous environmental pollutants widely used as plasticizers, which have been shown to interfere with both endocrine regulation and development of reproductive organs. In the present study, we examined the impact of diethylhexyl phthalate (DEHP) and dibutyl phthalate (DBP) on the proliferation of androgen-sensitive human prostate carcinoma LNCaP cells and related events. The results showed that both compounds were able to inhibit cell cycle progression in a dose-dependent manner. However, only DEHP was found to weakly reduce androgen receptor (AR) protein levels after long-term exposure, while only DBP partially inhibited expression of the prostate-specific antigen (KLK3) gene, a model AR transcriptional target. This indicated that inhibition of cell proliferation was likely independent of any AR modulations. Both phthalates induced suppression of cell proliferation, but none of them affected the levels of markers associated with neuroendocrine transdifferentiation (NED) in LNCaP cells.
Permanent Link: http://hdl.handle.net/11104/0239096