Antioxidant, antimicrobial and neutrophil-modulating activities of herb extracts

0435081 - BFÚ 2015 RIV PL eng J - Journal Article
Denev, P. - Kratchanova, M. - Číž, Milan - Lojek, Antonín - Vašíček, Ondřej - Blazheva, D. - Nedelcheva, P. - Vojtek, L. - Hyršl, P.
Antioxidant, antimicrobial and neutrophil-modulating activities of herb extracts.
Acta Biochimica Polonica. Roč. 61, č. 2 (2014), s. 359-367. ISSN 0001-527X. E-ISSN 1734-154X
Institutional support: RVO:68081707
Keywords : herbs * polyphenols * antioxidant activity
Subject RIV: BO - Biophysics
Impact factor: 1.153, year: 2014

The present study provides a comprehensive data on the antioxidant, antimicrobial and neutrophil-modulating activities of extracts from six medicinal plants - blackberry (Rubus fruticosus) leaves, chokeberry (Aronia melanocarpa) leaves, hawthorn (Crataegus monogyna) leaves, lady's mantle (Alchemilla glabra) aerial parts, meadowsweet (Filipendula ulmaria) aerial parts and raspberry (Rubus idaeus) leaves. In order to analyze the antioxidant activity of the herbs, several methods (ORAC, TRAP, HORAC and inhibition of lipid peroxidation) were used. Blackberry leaves and meadowsweet extracts revealed the highest antioxidant activities via all methods. All extracts studied blocked almost completely the opsonized zymosan particle-activated ROS production by neutrophils from human whole blood. On the other hand, the effect of extracts on phorbol myristate acetate-activated ROS production was much milder and even nonsignificant in the case of chokeberry leaves. This latter result suggests that extracts (apart from their antioxidative activity) interfere with the signaling cascade of phagocyte activation upstream of the protein kinase C activation.
Permanent Link: http://hdl.handle.net/11104/0239068