Monitoring the influence of antibiotic exposure using Raman spectroscopy

0434960 - ÚPT 2015 RIV US eng C - Conference Paper (international conference)
Samek, Ota - Zemánek, Pavel - Bernatová, Silvie - Ježek, Jan - Šerý, Mojmír - Jákl, Petr - Šiler, Martin - Růžička, F.
Monitoring the influence of antibiotic exposure using Raman spectroscopy.
Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry (Proceedings of SPIE 8939). Bellingham: SPIE, 2014, 89390P-1-6. ISBN 9780819498526. ISSN 0277-786X.
[Biomedical Vibrational Spectroscopy /6./: Advances in Research and Industry. San Francisco (US), 01.02.2014-02.02.2014]
R&D Projects: GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01; GA ČR GAP205/11/1687
Institutional support: RVO:68081731
Keywords : Raman spectroscopy * Fingerprint recognition * Principal component analysis
Subject RIV: BH - Optics, Masers, Lasers

Here we report on combination of the data obtained from MICs (minimum inhibitory concentrations) with infor- mation of microoragnisms fingerprint provided by Raman spectroscopy. In our feasibility study we could follow mechanisms of the bacteriostatic versus bactericidal action on biofilm-positive Staphylococcus epidermidis simply by monitoring Raman bands corresponding to DNA translating the changes introduced by selected antibiotics. The Raman spectra of Staphylococcus epidermidis treated with a bacteriostatic agent show little effect on DNA which is in contrast with the action of a bactericidal agent where decreased in dedicated Raman spectra signal strength suggests DNA fragmentation. Moreover, we demonstrate that Raman tweezers are indeed able to distinguish strains of biofilm-forming (biofilm-positive) and biofilm-negative Staphylococcus epidermidis strains using principal component analysis (PCA).
Permanent Link: http://hdl.handle.net/11104/0238899