Epithelial cells morphology and adhesion on diamonds films deposited and chemically modified by plasma processes

0434611 - FZÚ 2015 RIV DE eng J - Journal Article
Rezek, Bohuslav - Ukraintsev, Egor - Krátká, Marie - Taylor, Andrew - Fendrych, František - Mandys, V.
Epithelial cells morphology and adhesion on diamonds films deposited and chemically modified by plasma processes.
Biointerphases. Roč. 9, č. 3 (2014), "031012-1"-"031012-8". ISSN 1934-8630. E-ISSN 1559-4106
R&D Projects: GA ČR GAP108/12/0996; GA AV ČR KAN400100701
Institutional support: RVO:68378271
Keywords : nanocrystalline diamond (NCD) * AFM * surfaces * DNA
Subject RIV: BO - Biophysics
Impact factor: 3.374, year: 2014
http://scitation.aip.org/content/avs/journal/bip/9/3/10.1116/1.4890471

The authors show that nanocrystalline diamond (NCD) thin films prepared by microwave plasma enhanced chemical vapor deposition apparatus with a linear antenna delivery system are well compatible with epithelial cells (5637 human bladder carcinoma) and significantly improve the cell adhesion compared to reference glass substrates. This is attributed to better adhesion of adsorbed layers to diamond as observed by atomic force microscopy (AFM) beneath the cells. Moreover, the cell morphology can be adjusted by appropriate surface treatment of diamond by using hydrogen and oxygen plasma. Cell bodies, cytoplasmic rims, and filopodia were characterized by Peakforce AFM. Oxidized NCD films perform better than other substrates under all conditions (96% of cells adhered well). A thin adsorbed layer formed from culture medium and supplemented with fetal bovine serum (FBS) covered the diamond surface and played an important role in the cell adhesion.
Permanent Link: http://hdl.handle.net/11104/0238588