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Prince Cangrande’s Collagen: Study of Protein Modification on the Mummy of the Lord of Verona, Italy (1291–1329 AD)
- 1.0434566 - FGÚ 2015 RIV DE eng J - Journal Article
Mikšík, Ivan - Sedláková, Pavla - Pataridis, Statis - Bortolotti, F. - Gottardo, R. - Tagliaro, F.
Prince Cangrande’s Collagen: Study of Protein Modification on the Mummy of the Lord of Verona, Italy (1291–1329 AD).
Chromatographia. Roč. 77, 21-22 (2014), s. 1503-1510. ISSN 0009-5893. E-ISSN 1612-1112
R&D Projects: GA ČR(CZ) GAP206/12/0453; GA ČR(CZ) GA13-17224S
Institutional support: RVO:67985823
Keywords : HPLC/MS * collagen * deamidation * mummy * protein modification
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 1.411, year: 2014
The natural mummy of prince Cangrande, Lord of Verona, Italy (1291–1329 AD) was studied. The samples were cleaved with trypsin and analysed by liquid chromatographic methods coupled to mass spectrometry (Q-TOF, ion-trap). Special attention was devoted to nonenzymatic protein modification––the deamidation of asparagine and glutamine. A huge amount of collagen was determined in the tissues of the mummy (covering over 80 % of the sequence)––collagen type I was identified in the rib bone and collagen types I and III in the muscle. A high overall percentage of asparaginyl and glutaminyl residues were deamidated (up to 92 %)
Permanent Link: http://hdl.handle.net/11104/0238600
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