An Environmental Scanning Electron Microscopy and Observation if Live Nature

0434486 - ÚPT 2015 JP eng A - Abstract
Neděla, Vilém - Tihlaříková, Eva - Shiojiri, M.
An Environmental Scanning Electron Microscopy and Observation if Live Nature.
10th Japanese-Polish Joint Seminar on Micro and Nano Analysis. Abstract Book. Sapporo: -, 2014. s. 12-13.
[Japanese-Polish Joint Seminar on Micro and Nano Analysis /10./. 24.10.2014-26.10.2014, Sapporo]
R&D Projects: GA ČR(CZ) GA14-22777S
Institutional support: RVO:68081731
Keywords : SEM * observation of live nature

Observation of biological alive samples is one of the biggest dreams of electron microscopy. However, biological samples are commonly chemically fixed and dried, sputter-coated on their surfaces with thin conductive film or rapidly frozen, and observed by electron irradiation under vacuum in the conventional transmission electron microscopes or scanning electron microscopes . For overcoming the problem of charging and approaching the possibility to observe samples in their nature state, scientists have tried to use a gas environment in electron microscopes such as environmental scanning electron microscope, and many results of samples observation in various environmental atmospheres were reported. Unfortunately, they have not succeeded in the observing biological specimens alive. Here we show that our ESEM has in-situ observed a live animal.
Permanent Link: http://hdl.handle.net/11104/0238519