Three-dimensional microscopic analysis of SERS-active substrates for ultrasensitive detection of biologically significant compounds

0434467 - FZÚ 2015 CZ eng A - Abstract
Štolcová, L. - Ižák, Tibor - Petrenec, M. - Svoboda, M. - Proška, J. - Procházka, M. - Kromka, Alexander
Three-dimensional microscopic analysis of SERS-active substrates for ultrasensitive detection of biologically significant compounds.
18th International Microscopy Congress Proceedings. Praha: Czechoslovak microscopy society, 2014 - (Hozák, P.). ISBN 978-80-260-6720-7.
[International Microscopy Congress /18./. 07.09.2014-12.09.2014, Praha]
Institutional support: RVO:68378271
Keywords : surface-enhanced Raman scattering * close-packed polystyrene microspheres * FIB-FESEM
Subject RIV: BM - Solid Matter Physics ; Magnetism

Periodic arrays of close-packed polystyrene microspheres (diameters 250 nm - 4 um) were prepared using a self-assembly technique, and deep corrugations were created in the sphere surface by oxygen plasma etching. The corrugated sphere arrays coated with 20 nm of gold by magnetron sputtering displayed excellent SERS signal uniformity and low detection limits for a series of biologically significant compounds with different lipophilicity. SERS spectra of thiaclopridwere measured down to concentration of 10-8 M. The SERS signal uniformity was demonstrated by spectral mapping using Horiba JobinYvon LabRam HR800 Raman microspetrometer.
Permanent Link: http://hdl.handle.net/11104/0238510