Effect of zearalenone on reproductive parameters and expression of selected testicular genes in mice

0434230 - BTÚ 2015 RIV NL eng J - Journal Article
Žatecká, Eva - Děd, Lukáš - Elzeinová, Fatima - Kubátová, Alena - Dorosh, Andriy - Margaryan, Hasmik - Dostálová, Pavla - Korenková, Vlasta - Hošková, K. - Pěknicová, Jana
Effect of zearalenone on reproductive parameters and expression of selected testicular genes in mice.
Reproductive Toxicology. Roč. 45, june (2014), s. 20-30. ISSN 0890-6238. E-ISSN 1873-1708
R&D Projects: GA ČR(CZ) GAP503/12/1834; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional research plan: CEZ:AV0Z50520701
Institutional support: RVO:86652036
Keywords : zearalenone * fertility * reproductive parameters in male mice * spermatogenesis * gene expression * qPCR
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.227, year: 2014

We tested the effect of two different concentrations (150μg/l and 0.15μg/l) of mycotoxin zearalenone (ZEA) on the reproductive parameters and expression of testicular genes in male mice. In adult males, no reduction of body or reproductive organ weight was observed, and the seminiferous tubules were morphologically normal with ongoing spermatogenesis. However, we found decreased sperm concentration, increase of morphologically abnormal spermatozoa and increased binding of apoptotic marker annexin V. This study was also focused on the evaluation of gene expression profiles of 28 genes playing important roles during the processes occurring in the testicular tissue. We detected changes in the expression of genes important for proper spermatogenesis. Surprisingly, we observed a stronger effect after exposure to the lower dose of ZEA.
Permanent Link: http://hdl.handle.net/11104/0238381