Macroporous cryogel based spin column with immobilized concanavalin A for isolation of glycoproteins

0434034 - ÚIACH 2016 RIV DE eng J - Journal Article
Křenková, Jana - Česla, P. - Foret, František
Macroporous cryogel based spin column with immobilized concanavalin A for isolation of glycoproteins.
Electrophoresis. Roč. 36, 11-12 (2015), s. 1344-1348. ISSN 0173-0835. E-ISSN 1522-2683
R&D Projects: GA ČR(CZ) GA14-06319S
Grant - others:GA AV ČR(CZ) M200311201
Institutional support: RVO:68081715
Keywords : concanavalin A * cryogel * monolith
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 2.482, year: 2015

In this work we have developed a hydrophilic poly(hydroxyethyl methacrylate-co-poly(ethylene glycol) diacrylate) cryogel placed in the centrifugal filter device. The surface of the monolithic scaffold was activated by epichlorohydrin and used for immobilization of concanavalin A to provide the affinity supports for selective isolation of glycoproteins.
Permanent Link: http://hdl.handle.net/11104/0238174