0433504 - ÚOCHB 2015 RIV DE eng J - Journal Article
Hnízda, Aleš - Skleničková, Radka - Pachl, Petr - Fábry, Milan - Tošner, Z. - Brynda, Jiří - Veverka, Václav
Backbone resonance assignments of human cytosolic dNT-1 nucleotidase.
Biomolecular NMR Assignments. Roč. 8, č. 2 (2014), s. 425-428. ISSN 1874-2718. E-ISSN 1874-270X
R&D Projects: GA MŠMT(CZ) LK11205; GA ČR GA203/09/0820
Institutional support: RVO:61388963 ; RVO:68378050
Keywords : 5 '-nucleotidase * haloacid dehalogenase superfamily * backbone assignments * NMR * perdeuterated protein * dimer * pyrimidine nucleotides
Subject RIV: CE - Biochemistry; EB - Genetics ; Molecular Biology (UMG-J)
Impact factor: 0.760, year: 2014 ; AIS: 0.234, rok: 2014
DOI: https://doi.org/10.1007/s12104-013-9531-1

Cytosolic dNT-1 nucleotidase plays a key role in the homeostasis of pyrimidine deoxyribonucleotides in mammalian cells. The enzyme is responsible for the dephosphorylation of physiological substrates as well as nucleoside analogues that are used in antiviral and anticancer therapies, therefore selective inhibition of the dNT-1 nucleotidase activity may lead to an increase in efficacy of this type of therapeutic compounds. Here, we report the backbone H-1, C-13 and N-15 assignments for the 47 kDa dNT-1 dimer, which will be used for structural characterisation of dNT-1 complexes with small molecule inhibitors obtained through modification of pyrimidine nucleotide scaffolds or optimisation of successful binders obtained from the screening of fragment libraries.
Permanent Link: http://hdl.handle.net/11104/0237785