Effects of mtDNA in SHR-mtF344 versus SHR conplastic strains on reduced OXPHOS enzyme levels, insulin resistance, cardiac hypertrophy, and systolic dysfunction

0433305 - FGÚ 2015 RIV US eng J - Journal Article
Houštěk, Josef - Vrbacký, Marek - Hejzlarová, Kateřina - Zídek, Václav - Landa, Vladimír - Šilhavý, Jan - Šimáková, Miroslava - Mlejnek, Petr - Kazdová, L. - Mikšík, Ivan - Neckář, Jan - Papoušek, František - Kolář, František - Kurtz, T. W. - Pravenec, Michal
Effects of mtDNA in SHR-mtF344 versus SHR conplastic strains on reduced OXPHOS enzyme levels, insulin resistance, cardiac hypertrophy, and systolic dysfunction.
Physiological Genomics. Roč. 46, č. 18 (2014), s. 671-678. ISSN 1094-8341. E-ISSN 1531-2267
R&D Projects: GA MŠMT(CZ) LL1204; GA ČR(CZ) GB14-36804G; GA ČR(CZ) GA13-10267S; GA MŠMT(CZ) 7E10067
Institutional support: RVO:67985823
Keywords : SHR conplastic strain with F344 mtDNA * impaired glucose tolerance * systolic dysfunction
Subject RIV: FB - Endocrinology, Diabetology, Metabolism, Nutrition
Impact factor: 2.374, year: 2014

In the current study, we investigated the metabolic and hemodynamic effects of the SHR versus F344 mtDNA by comparing the SHR versus SHR-mtF344 conplastic strains that are genetically identical except for their mitochondrial genomes. Altogether 13 amino acid substitutions in protein coding genes, 7 single nucleotide polymorphisms in tRNA genes and 12 single nucleotide changes in rRNA genes were detected in F344 mtDNA when compared to SHR mtDNA. Analysis of oxidative phosphorylation system (OXPHOS) in heart left ventricles (LV), muscle and liver revealed reduced activity and content of several respiratory chain complexes in SHR-mtF344 conplastic rats when compared to the SHR strain. Lower function of OXPHOS in LV of conplastic rats was associated with significantly increased relative ventricular mass and reduced fractional shortening which was independent of blood pressure
Permanent Link: http://hdl.handle.net/11104/0237598