Modulation of the Early Inflammatory Microenvironment in the Alkali-Burned Eye by Systemically Administered Interferon-γ-Treated Mesenchymal Stromal Cells

0432995 - ÚEM 2015 RIV GB eng J - Journal Article
Javorková, Eliška - Trošan, Peter - Zajícová, Alena - Krulová, Magdaléna - Hájková, M. - Holáň, Vladimír
Modulation of the Early Inflammatory Microenvironment in the Alkali-Burned Eye by Systemically Administered Interferon-γ-Treated Mesenchymal Stromal Cells.
Stem Cells and Development. Roč. 23, č. 20 (2014), s. 2490-2500. ISSN 1547-3287. E-ISSN 1557-8534
R&D Projects: GA ČR GAP304/11/0653; GA ČR(CZ) GAP301/11/1568; GA ČR GA14-12580S; GA MZd NT14102
Grant - others:GA UK(CZ) 668012; GA UK(CZ) 889113; GA UK(CZ) 546613
Institutional support: RVO:68378041
Keywords : mesenchymal stromal cells * nitric oxide * transforming growth factor beta
Subject RIV: EC - Immunology
Impact factor: 3.727, year: 2014

The aim of this study was to investigate the effects of systemically administered bone-marrow-derived mesenchymal stromal cells (MSCs) on the early acute phase of inflammation in the alkali-burned eye. Mice with damaged eyes were either untreated or treated 24?h after the injury with an intravenous administration of fluorescent-dye-labeled MSCs that were unstimulated or pretreated with interleukin-1α (IL-1α), transforming growth factor-β (TGF-β), or interferon-γ (IFN-γ). Analysis of cell suspensions prepared from the eyes of treated mice on day 3 after the alkali burn revealed that MSCs specifically migrated to the damaged eye and that the number of labeled MSCs was more than 30-times higher in damaged eyes compared with control eyes. The study of the composition of the leukocyte populations within the damaged eyes showed that all types of tested MSCs slightly decreased the number of infiltrating lymphoid and myeloid cells, but only MSCs pretreated with IFN-γ significantly decreased the percentage of eye-infiltrating cells with a more profound effect on myeloid cells.
Permanent Link: http://hdl.handle.net/11104/0239360