Plant Cell Morphogenesis: Methods and Protocols

0432114 - ÚEB 2015 RIV US eng M - Monography Chapter
Seifertová, Daniela - Klíma, Petr - Pařezová, Markéta - Petrášek, Jan - Zažímalová, Eva - Opatrný, Z.
Plant Cell Lines in Cell Morphogenesis Research.
Plant Cell Morphogenesis: Methods and Protocols. Vol. 1080. New York: Humana Press, 2014 - (Žárský, V.; Cvrčková, F.), s. 215-229. Methods in Molecular Biology. ISBN 978-1-62703-643-6
R&D Projects: GA ČR(CZ) GAP305/11/0797; GA ČR(CZ) GAP305/11/2476
Institutional support: RVO:61389030
Keywords : BY-2 * VBI-0 * Suspension-cultured cells
Subject RIV: EB - Genetics ; Molecular Biology

Plant organs and tissues consist of many various cell types, often in different phases of their development. Such complex structures do not allow direct studies on behavior of individual cells. In contrast, populations of in vitro-cultured plant cells represent valuable tool for studying processes on a single-cell level, including cell morphogenesis. Here we describe characteristics of well-established model tobacco and Arabidopsis cell lines and provide detailed protocol on their cultivation, characterization, and genetic transformation.
Permanent Link: http://hdl.handle.net/11104/0236577