Plant Cell Morphogenesis: Methods and Protocols

0432113 - ÚEB 2015 RIV US eng M - Monography Chapter
Cvrčková, F. - Hála, Michal
Heterologous Expression in Budding Yeast as a Tool for Studying the Plant Cell Morphogenesis Machinery.
Plant Cell Morphogenesis: Methods and Protocols. Vol. 1080. New York: Humana Press, 2014 - (Žárský, V.; Cvrčková, F.), s. 267-282. Methods in Molecular Biology. ISBN 978-1-62703-643-6
Institutional support: RVO:61389030
Keywords : Saccharomyces cerevisiae * Heterologous gene expression * Inducible expression system
Subject RIV: EB - Genetics ; Molecular Biology

The budding yeast (Saccharomyces cerevisiae) can serve as a unique experimental system for functional studies of heterologous genes, allowing not only complementation of readily available yeast mutations but also generation of overexpression phenotypes and in some cases also rescue of such phenotypes. Here we summarize the main considerations that have to be taken into account when using the yeast expression system for investigating the function of plant genes participating in cell morphogenesis; outline the strategies of experiment planning, yeast strain selection (or construction), and expression vector choice; and provide detailed protocols for yeast transformation, transformant selection, and phenotype evaluation.
Permanent Link: http://hdl.handle.net/11104/0236576