Plant Cell Morphogenesis: Methods and Protocols

0432112 - ÚEB 2015 RIV US eng M - Monography Chapter
Bezvoda, Radek - Pleskot, Roman - Žárský, Viktor - Potocký, Martin
Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes.
Plant Cell Morphogenesis: Methods and Protocols. Vol. 1080. New York: Humana Press, 2014 - (Žárský, V.; Cvrčková, F.), s. 231-236. Methods in Molecular Biology. ISBN 978-1-62703-643-6
R&D Projects: GA ČR GA13-19073S
Institutional support: RVO:61389030
Keywords : Antisense oligodeoxynucleotide * Pollen tube * AODN
Subject RIV: EB - Genetics ; Molecular Biology

Specific gene knockdown mediated by the antisense oligodeoxynucleotides (AODNs) strategy recently emerged as a rapid and effective tool for probing gene role in plant cells, particularly tip-growing pollen tubes. Here, we describe the protocol for the successful employment of AODN technique in growing tobacco pollen tubes, covering AODN design, application, and analysis of the results. We also discuss the advantages and drawbacks of this method.
Permanent Link: http://hdl.handle.net/11104/0236575