Laser-induced fluorescence spectroscopy in tissue local necrosis detection

0431931 - ÚPT 2015 RIV US eng C - Conference Paper (international conference)
Číp, Ondřej - Buchta, Zdeněk - Lešundák, Adam - Randula, A. - Mikel, Břetislav - Lazar, Josef - Veverková, L.
Laser-induced fluorescence spectroscopy in tissue local necrosis detection.
Optical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications (Proceedings of SPIE 8941). Bellingham: SPIE, 2014, 89411D:1-6. ISSN 1605-7422.
[Optical Interactions with Tissue and Cells /25./; and Terahertz for Biomedical Applications. San Francisco (US), 02.02.2014-04.02.2014]
R&D Projects: GA ČR GAP102/10/1813; GA MŠMT EE2.4.31.0016; GA MŠMT ED0017/01/01; GA MŠMT(CZ) LO1212
Institutional support: RVO:68081731
Keywords : IndoCyanine Green * fluorescence spectroscopy * small intestine * surgery
Subject RIV: BH - Optics, Masers, Lasers

The recent effort leads to reliable imaging techniques which can help to a surgeon during operations. The fluorescence spectroscopy was selected as very useful online in vivo imaging method to organics and biological materials analysis. The presented work scopes to a laser induced fluorescence spectroscopy technique to detect tissue local necrosis in small intestine surgery. In first experiments, we tested tissue auto-fluorescence technique but a signal-to-noise ratio didn't express significant results. Then we applied a contrast dye - IndoCyanine Green (ICG) which absorbs and emits wavelengths in the near IR. We arranged the pilot experimental setup based on highly coherent extended cavity diode laser (ECDL) used for stimulating of some critical areas of the small intestine tissue with injected ICG dye. We demonstrated the distribution of the ICG exciter with the first file of shots of small intestine tissue of a rabbit that was captured by high sensitivity fluorescent cam.
Permanent Link: http://hdl.handle.net/11104/0236454