GABA(B) receptor phosphorylation regulates KCTD12-induced K+ current desensitization

0431570 - ÚEM 2015 RIV US eng J - Journal Article
Adelfinger, L. - Tureček, Rostislav - Ivankova, K. - Jensen, A. A. - Moss, S. J. - Gassmann, M. - Bettler, B.
GABA(B) receptor phosphorylation regulates KCTD12-induced K+ current desensitization.
Biochemical Pharmacology. Roč. 91, č. 3 (2014), s. 369-379. ISSN 0006-2952. E-ISSN 1873-2968
Institutional support: RVO:68378041
Keywords : GABA-B * G-protein coupled receptor * GPCR
Subject RIV: FH - Neurology
Impact factor: 5.009, year: 2014

We studied modulation of KCTD12-induced desensitization of GABAB receptor activated K+ currents by protein kinase A (PKA). We found that PKA phosphorylation of S892 on the C-tail of GB2 subunit reduces KCTD12-induced desensitization. Mechanistically, S892 phosphorylation induces a conformational change in the GABAB receptor/KCTD12 complex that slows KCTD12-induced desensitization. Reciprocally, the binding of KCTD12 promotes phosphorylation of S892, consistent with previous data showing that KCTD12 stabilizes receptors at the cell surface.
Permanent Link: http://hdl.handle.net/11104/0238719