PLANT CHEMICAL GENOMICS: METHODS AND PROTOCOLS

0430550 - ÚEB 2015 RIV US eng M - Monography Chapter
Simon, Sibu - Skůpa, Petr - Dobrev, Petre - Petrášek, Jan - Zažímalová, Eva - Friml, J.
Analyzing the In Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters.
PLANT CHEMICAL GENOMICS: METHODS AND PROTOCOLS. Totowa: Humana Press, 2014 - (Hicks, G.; Robert, S.), s. 255-264. Methods in Molecular Biology. ISBN 978-1-62703-591-0
R&D Projects: GA ČR(CZ) GAP305/11/0797
Institutional support: RVO:61389030
Keywords : Auxin * Auxin metabolism * BY-2 tobacco cells
Subject RIV: EB - Genetics ; Molecular Biology

Exogenous application of biologically important molecules for plant growth promotion and/or regulation is very common both in plant research and horticulture. Plant hormones such as auxins and cytokinins are classes of compounds which are often applied exogenously. Nevertheless, plants possess a well-established machinery to regulate the active pool of exogenously applied compounds by converting them to metabolites and conjugates. Consequently, it is often very useful to know the in vivo status of applied compounds to connect them with some of the regulatory events in plant developmental processes. The in vivo status of applied compounds can be measured by incubating plants with radiolabeled compounds, followed by extraction, purification, and HPLC metabolic profiling of plant extracts. Recently we have used this method to characterize the intracellularly localized PIN protein, PIN5. Here we explain the method in detail, with a focus on general application.
Permanent Link: http://hdl.handle.net/11104/0235508