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Polymerase Synthesis of Photocaged DNA Resistant against Cleavage by Restriction Endonucleases
- 1.0429964 - ÚOCHB 2015 RIV DE eng J - Journal Article
Vaníková, Zuzana - Hocek, Michal
Polymerase Synthesis of Photocaged DNA Resistant against Cleavage by Restriction Endonucleases.
Angewandte Chemie - International Edition. Roč. 53, č. 26 (2014), s. 6734-6737. ISSN 1433-7851. E-ISSN 1521-3773
R&D Projects: GA ČR GA14-04289S
Institutional support: RVO:61388963
Keywords : DNA * DNA polymerase * nucleotides * photocaging * protective groups
Subject RIV: CC - Organic Chemistry
Impact factor: 11.261, year: 2014
5-[(2-Nitrobenzyl) oxymethyl]-2'-deoxyuridine 5'-O-triphosphate was used for polymerase (primer extension or PCR) synthesis of photocaged DNA that is resistant to the cleavage by restriction endonucleases. Photodeprotection of the caged DNA released 5-hydroxymethyluracil-modified nucleic acids, which were fully recognized and cleaved by restriction enzymes.
Permanent Link: http://hdl.handle.net/11104/0234976
Number of the records: 1