Phosphoproteomic studies in Arabidopsis and tobacco male gametophytes

0429936 - ÚEB 2015 RIV GB eng J - Journal Article
Fíla, Jan - Čapková, Věra - Honys, David
Phosphoproteomic studies in Arabidopsis and tobacco male gametophytes.
Biochemical Society Transactions. Roč. 42, č. 2 (2014), s. 383-387. ISSN 0300-5127. E-ISSN 1470-8752
R&D Projects: GA ČR(CZ) GAP501/11/1462; GA ČR(CZ) GAP305/12/2611; GA ČR GA13-06943S; GA MŠMT(CZ) LD13049
Institutional support: RVO:61389030
Keywords : Arabidopsis thaliana * male gametophyte * mature pollen
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.194, year: 2014

Mature pollen represents an extremely resistant quiescent structure surrounded by a tough cell wall. After its hydration on stigma papillary cells, pollen tube growth starts rapidly. Massive metabolic changes are likely to be accompanied by changes in protein phosphorylation. Protein phosphorylation belongs among the most rapid post-translational modifications. To date, only Arabidopsis thaliana and tobacco (Nicotiana tabacum) mature pollen have been subjected to phosphoproteomic studies in order to identify the phosphoproteins present. In the present mini-review, Arabidopsis and tobacco datasets were compared with each other. The representation of the O-phosphorylated amino acids was compared between these two datasets, and the putative pollen-specific or pollen-abundant phosphopeptides were highlighted. Finally, the phosphorylation sites common for both Arabidopsis and tobacco phosphoproteins are listed as well as the phosphorylation motifs identified.
Permanent Link: http://hdl.handle.net/11104/0234900