Recent developments in capillary and microchip electroseparations of peptides (2011-2013)

0429826 - ÚOCHB 2015 RIV DE eng J - Journal Article
Kašička, Václav
Recent developments in capillary and microchip electroseparations of peptides (2011-2013).
Electrophoresis. Roč. 35, č. 1 (2014), s. 69-95. ISSN 0173-0835. E-ISSN 1522-2683
R&D Projects: GA ČR(CZ) GAP206/12/0453; GA ČR(CZ) GA13-17224S
Institutional support: RVO:61388963
Keywords : capillary electrochromatography * capillary electrophoresis * microchip * peptides * proteins
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 3.028, year: 2014

The review presents a comprehensive survey of recent developments and applications of capillary and microchip electroseparation methods (zone electrophoresis, ITP, IEF, affinity electrophoresis, EKC, and electrochromatography) for analysis, isolation, purification, and physicochemical and biochemical characterization of peptides. Advances in the investigation of electromigration properties of peptides, in the methodology of their analysis, including sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, as well as in detection of peptides, are presented. New developments in particular CE and CEC modes are reported and several types of their applications to peptide analysis are described: conventional qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC techniques to provide relevant physicochemical characteristics of peptides are demonstrated.
Permanent Link: http://hdl.handle.net/11104/0234951