Statistical filtering in fluorescence microscopy and fluorescence correlation spectroscopy

0429656 - ÚFCH JH 2015 RIV DE eng J - Journal Article
Macháň, Radek - Kapusta, Peter - Hof, Martin
Statistical filtering in fluorescence microscopy and fluorescence correlation spectroscopy.
Analytical and Bioanalytical Chemistry. Roč. 406, č. 20 (2014), s. 4797-4813. ISSN 1618-2642. E-ISSN 1618-2650
R&D Projects: GA ČR GBP208/12/G016
Institutional support: RVO:61388955
Keywords : Filtered fluorescence correlation spectroscopy * Fluorescence lifetime correlation spectroscopy * Fluorescence spectral correlation spectroscopy
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 3.436, year: 2014

We review the principles and applications of statistical filtering in multichannel fluorescence microscopy. This alternative approach to separation of signals from individual fluorophore populations has many important advantages, especially when spectral and/or temporal overlap, or the complicated nature of those signals, makes their discrimination or sorting impossible by means of hardware. This situation is typically encountered for biological samples. This review of well established statistical filtering techniques and of emerging, very promising new methods of analysis reveals remarkable progress in bioanalytical applications of fluorescence microscopy.
Permanent Link: http://hdl.handle.net/11104/0234737