Bacteria dominate ammonia oxidation in soils used for outdoor cattle overwintering

0429115 - BC 2015 RIV NL eng J - Journal Article
Radl, V. - Chroňáková, Alica - Čuhel, Jiří - Šimek, Miloslav - Elhottová, Dana - Welzl, G. - Schloter, M.
Bacteria dominate ammonia oxidation in soils used for outdoor cattle overwintering.
Applied Soil Ecology. Roč. 77, May (2014), s. 68-71. ISSN 0929-1393. E-ISSN 1873-0272
R&D Projects: GA MŠMT LC06066
Grant - others:Akademie věd ČR(CZ) D-CZ 45:05/06
Institutional support: RVO:60077344
Keywords : ammonia oxidation * bacteria * archaea * amoA diversity * urea * pasture
Subject RIV: EH - Ecology, Behaviour
Impact factor: 2.644, year: 2014

In areas used for cattle overwintering detrimental effects normally associated with grazing are intensified. Among the alterations observed, increases on the N availability and soil pH may highly influence structure of ammonia oxidizing microbes and thus influence nitrification pattern in soil. To evaluate this assumption, we assessed the abundance and diversity of ammonia oxidizing bacteria (AOB) and archaea (AOA) in three sites with different degrees of animal impact (severe, moderate or no impact) of an overwintering pasture by means of qPCR and T-RFLP of amoA genes. In areas where no animal impact could be identified AOA was dominating over AOB. However, AOB abundance increased as the degree of animal impact enhances, becoming most dominant in the severely impacted site. Interestingly, the diversity of AOB was the highest in the severely impacted area, where AOA diversity was the lowest. Obviously the pressure imposed by altered environmental conditions created by cattle husbandry lead to the selection of AOB and AOA populations, adapted to alkaline pH and higher ammonia concentration.
Permanent Link: http://hdl.handle.net/11104/0236130