Study of DNA-protein interactions by cross-link formation using aqueous Michael addition

0428937 - ÚOCHB 2015 CZ eng C - Conference Paper (international conference)
Daďová, Jitka - Orság, Petr - Pohl, Radek - Brázdová, Marie - Fojta, Miroslav - Hocek, Michal
Study of DNA-protein interactions by cross-link formation using aqueous Michael addition.
Chemistry of Nucleic Acid Components. 16th Symposium. Praha: Institute of Organic Chemistry and Biochemistry AS CR, v. v. i, 2014 - (Hocek, M.), s. 240-241. Collection Symposium Series, 14. ISBN 978-80-86241-50-0.
[Symposium on Chemistry of Nucleic Acid Components /16./. Český Krumlov (CZ), 08.06.2014-13.06.2014]
R&D Projects: GA ČR GA203/09/0317; GA ČR(CZ) GAP301/11/2076
Institutional support: RVO:61388963 ; RVO:68081707
Keywords : nucleic acids * DNA * Michael addition
Subject RIV: CC - Organic Chemistry; BO - Biophysics (BFU-R)

Acrylamide and vinylsulfonamide were designed as reactive DNA modifications for covalent crosslinking with cysteine containing peptides and proteins. The corresponding functionalized cytidine triphosphates were prepared and enzymatically incorporated into DNA. These modified oligonucleotides were then used for conjugation with DNA binding domain of protein p53.
Permanent Link: http://hdl.handle.net/11104/0234284