Polymerase synthesis of base-modified DNA: New methods and new applications

0428805 - ÚOCHB 2015 CZ eng C - Conference Paper (international conference)
Balintová, Jana - Daďová, Jitka - Kielkowski, Pavel - Ménová, Petra - Vaníková, Zuzana - Riedl, Jan - Raindlová, Veronika - Fojta, Miroslav - Hocek, Michal
Polymerase synthesis of base-modified DNA: New methods and new applications.
Chemistry of Nucleic Acid Components. 16th Symposium. Praha: Institute of Organic Chemistry and Biochemistry AS CR, v. v. i, 2014 - (Hocek, M.), s. 66-67. Collection Symposium Series, 14. ISBN 978-80-86241-50-0.
[Symposium on Chemistry of Nucleic Acid Components /16./. Český Krumlov (CZ), 08.06.2014-13.06.2014]
R&D Projects: GA ČR GA203/09/0317; GA ČR GBP206/12/G151; GA ČR GA14-04289S
Institutional support: RVO:61388963 ; RVO:68081707
Keywords : nucleic acids * base-modified DNA * dNTPs
Subject RIV: CC - Organic Chemistry; BO - Biophysics (BFU-R)

Diverse base-modified oligonucleotides and double-stranded DNA molecules were prepared by polymerase incorporation of modified nucleoside triphosphates. The methods include primer extension, PCR, nicking enzyme amplification reaction and mixed incorporations. The modified nucleic acids were used in redox and fluorescence labelling and coding, as well as regulation of binding and cross-linking with proteins.
Permanent Link: http://hdl.handle.net/11104/0234074