Electrochemistry as a Tool for an Enzyme Characterization

0428758 - ÚFCH JH 2015 RIV CZ eng C - Conference Paper (international conference)
Gál, M. - Krahulec, J. - Jiríčková, K. - Sokolová, Romana - Híveš, J.
Electrochemistry as a Tool for an Enzyme Characterization.
Sborník přednášek mezinárodní odborné konference XXXIV. Moderní Elektrochemické Metody. Ústí nad Labem: Best Servis, 2014 - (Navrátil, T.; Fojta, M.; Pecková, K.), s. 40-43. ISBN 978-80-905221-2-1.
[Moderní Elektrochemické Metody /34./. Jetřichovice (CZ), 19.05.2014-23.05.2014]
Institutional support: RVO:61388955
Keywords : electrochemistry * Enterokinase * Impedance spectroscopy
OECD category: Physical chemistry

Basic biochemical properties such as Michaelis constant (KM) and turnover number (kcat) of enzyme enteropeptidase was measured by electrochemical impedance spectroscopy. Both values determined by impedance measurements are in good agreement with those obtained by traditional spectroscopic techniques. Therefore one can suppose that electrochemical methods might be successful for such measurements also in the case where usual ones (UV-Vis, fluorescence spectroscopy) are not able to determine these enzyme characteristics.
Permanent Link: http://hdl.handle.net/11104/0234055