Number of the records: 1  

The structure of metallo-DNA with consecutive thymine-Hg-II-thymine base pairs explains positive entropy for the metallo base pair formation

  1. 1.
    0428654 - ÚOCHB 2015 RIV GB eng J - Journal Article
    Yamaguchi, H. - Šebera, Jakub - Kondo, J. - Oda, S. - Komuro, T. - Kawamura, T. - Dairaku, T. - Kondo, Y. - Okamoto, I. - Ono, A. - Burda, J. V. - Kojima, C. - Sychrovský, Vladimír - Tanaka, Y.
    The structure of metallo-DNA with consecutive thymine-Hg-II-thymine base pairs explains positive entropy for the metallo base pair formation.
    Nucleic Acids Research. Roč. 42, č. 6 (2014), s. 4094-4099. ISSN 0305-1048. E-ISSN 1362-4962
    R&D Projects: GA ČR GAP205/10/0228
    Institutional support: RVO:61388963
    Keywords : NMR structures * mercury * metalation * metal-DNA binding * DFT
    Subject RIV: CF - Physical ; Theoretical Chemistry
    Impact factor: 9.112, year: 2014
    http://nar.oxfordjournals.org/content/42/6/4094.full

    We have determined the three-dimensional (3D) structure of DNA duplex that includes tandem Hg-II-mediated T-T base pairs (thymine-Hg-II-thymine, T-Hg-II-T) with NMR spectroscopy in solution. This is the first 3D structure of metallo-DNA (covalently metallated DNA) composed exclusively of 'NATURAL' bases. The T-Hg-II-T base pairs whose chemical structure was determined with the N-15 NMR spectroscopy were well accommodated in a B-form double helix, mimicking normal Watson-Crick base pairs. The Hg atoms aligned along DNA helical axis were shielded from the bulk water. The complete dehydration of Hg atoms inside DNA explained the positive reaction entropy (delta S) for the T-Hg-II-T base pair formation. The positive delta S value arises owing to the Hg-II dehydration, which was approved with the 3D structure. The 3D structure explained extraordinary affinity of thymine towards Hg-II and revealed arrangement of T-Hg-II-T base pairs in metallo-DNA.
    Permanent Link: http://hdl.handle.net/11104/0233994

     
     
Number of the records: 1  

  This site uses cookies to make them easier to browse. Learn more about how we use cookies.