Unbiased estimation of chloroplast number in mesophyll cells: advantage of a genuine three-dimensional approach

0428273 - FGÚ 2015 RIV GB eng J - Journal Article
Kubínová, Z. - Janáček, Jiří - Lhotáková, Z. - Kubínová, Lucie - Albrechtová, J.
Unbiased estimation of chloroplast number in mesophyll cells: advantage of a genuine three-dimensional approach.
Journal of Experimental Botany. Roč. 65, č. 2 (2014), s. 609-620. ISSN 0022-0957. E-ISSN 1460-2431
R&D Projects: GA ČR(CZ) GAP501/10/0340
Grant - others:Univerzita Karlova(CZ) SVV265203
Institutional research plan: CEZ:AV0Z50110509
Institutional support: RVO:67985823
Keywords : chloroplast counting * confocal microscopy * disector method * mesophyll * coniferous needle structure * Norway spruce (Picea abies L. Karst.), * profile counting * stereology
Subject RIV: GK - Forestry
Impact factor: 5.526, year: 2014

Comparing measurements of mesophyll cells, calculations performed in a 3D model of a cell with chloroplasts as well as a theoretical analysis showed that the frequently used 2D approach to counting of chloroplast number per mesophyll cell yielded biased results, while the underestimation could be up to 10-fold. The disector method can be efficiently used for unbiased estimation of chloroplast number per mesophyll cell. This should be the method of choice, especially in coniferous needles and leaves with mesophyll cells with lignified cell walls where maceration methods are difficult or impossible to use
Permanent Link: http://hdl.handle.net/11104/0233647