Lichen secondary metabolites as DNA-interacting agents

0427970 - BFÚ 2015 RIV GB eng J - Journal Article
Plšíková, J. - Štěpánková, Jana - Kašpárková, Jana - Brabec, Viktor - Bačkor, M. - Kozurková, M.
Lichen secondary metabolites as DNA-interacting agents.
Toxicology in Vitro. Roč. 28, č. 2 (2014), s. 182-186. ISSN 0887-2333. E-ISSN 1879-3177
Institutional support: RVO:68081707
Keywords : Lichens * DNA-binding * Topoisomerase I, II
Subject RIV: BO - Biophysics
Impact factor: 2.903, year: 2014

A series of lichen secondary metabolites (parietin, atranorin, usnic and gyrophoric acid) and their interactions with calf thymus DNA were investigated using molecular biophysics and biochemical methods. The binding constants K were estimated to range from 4.3 x 10(5) to 2.4 x 10(7) M-1 and the percentage of hypochromism was found to be 16-34% (from spectral titration). The results of spectral measurement indicate that the compounds act as effective DNA-interacting agents. Electrophoretic separation studies prove that from all the metabolites tested in this study, only gyrophoric acid exhibited an inhibitory effect on Topo I (25 mu M). (C) 2013 Elsevier Ltd. All rights reserved.
Permanent Link: http://hdl.handle.net/11104/0233410