Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase

0427960 - BFÚ 2015 RIV GB eng J - Journal Article
Schrumpfová, P. - Vychodilová, I. - Dvořáčková, Martina - Majerská, J. - Dokládal, Ladislav - Schorová, Š. - Fajkus, Jiří
Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase.
Plant Journal. Roč. 77, č. 5 (2014), s. 770-781. ISSN 0960-7412. E-ISSN 1365-313X
R&D Projects: GA MŠMT(CZ) ED1.1.00/02.0068; GA ČR(CZ) GA13-06943S
Institutional support: RVO:68081707
Keywords : telomere protein interaction * telomere repeat binding * Arabidopsis thaliana
Subject RIV: BO - Biophysics
Impact factor: 5.972, year: 2014

Although telomere-binding proteins constitute an essential part of telomeres, in vivo data indicating the existence of a structure similar to mammalian shelterin complex in plants are limited. Partial characterization of a number of candidate proteins has not identified true components of plant shelterin or elucidated their functional mechanisms. Telomere repeat binding (TRB) proteins from Arabidopsis thaliana bind plant telomeric repeats through a Myb domain of the telobox type in vitro, and have been shown to interact with POT1b (Protection of telomeres 1). Here we demonstrate co-localization of TRB1 protein with telomeres in situ using fluorescence microscopy, as well as in vivo interaction using chromatin immunoprecipitation. Classification of the TRB1 protein as a component of plant telomeres is further confirmed by the observation of shortening of telomeres in knockout mutants of the trb1 gene. Moreover, TRB proteins physically interact with plant telomerase catalytic subunits.
Permanent Link: http://hdl.handle.net/11104/0233396