Use of spent mushroom substrate for production of Bacillus thuringiensis by solid-state fermentation

0425083 - BC 2015 RIV US eng J - Journal Article
Wu, S. - Lan, Y. - Huang, D. - Peng, Y. - Huang, Z. - Xu, L. - Gelbič, Ivan - Carballar-Lejarazu, R. - Guan, X. - Zhang, L. - Zou, S.
Use of spent mushroom substrate for production of Bacillus thuringiensis by solid-state fermentation.
Journal of Economic Entomology. Roč. 107, č. 1 (2014), s. 137-143. ISSN 0022-0493. E-ISSN 1938-291X
Institutional support: RVO:60077344
Keywords : Bacillus thuringiensis * spent mushroom substrate * solid-state fermentation
Subject RIV: GF - Plant Pathology, Vermin, Weed, Plant Protection
Impact factor: 1.506, year: 2014
http://www.bioone.org/doi/pdf/10.1603/EC13276

The aim of this study was to explore a cost-effective method for the mass production of Bacillus thuringiensis (Bt) by solid-state fermentation. As a locally available agroindustrial byproduct, spent mushroom substrate (SMS) was used as raw material for Bt cultivation, and four combinations of SMS-based media were designed. Fermentation conditions were optimized on the best medium and the optimal conditions were determined as follows: temperature 32°C, initial pH value 6, moisture content 50%, the ratio of sieved material to initial material 1:3, and inoculum volume 0.5 ml. Large scale production of B. thuringiensis subsp. israelensis (Bti) LLP29 was conducted on the optimal medium at optimal conditions. High toxicity (1,487 international toxic units/milligram) and long larvicidal persistence of the product were observed in the study, which illustrated that SMS-based solid-state fermentation medium was efŢcient and economical for large scale industrial production of Bt-based biopesticides. The cost of production of 1 kg of Bt was approximately US$0.075.
Permanent Link: http://hdl.handle.net/11104/0233150