Strong Accumulation of Chloroplast DNA in the Y Chromosomes of Rumex acetosa and Silene latifolia

0424729 - BFÚ 2014 RIV CH eng J - Journal Article
Šteflová, Pavlína - Hobza, Roman - Vyskot, Boris - Kejnovský, Eduard
Strong Accumulation of Chloroplast DNA in the Y Chromosomes of Rumex acetosa and Silene latifolia.
Cytogenetic and Genome Research. Roč. 142, č. 1 (2014), s. 59-65. ISSN 1424-8581. E-ISSN 1424-859X
R&D Projects: GA ČR(CZ) GAP305/10/0930; GA ČR(CZ) GAP501/10/0102; GA ČR(CZ) GBP501/12/G090; GA ČR GAP501/12/2220; GA ČR(CZ) GA522/09/0083; GA MŠMT(CZ) LO1204
Institutional research plan: CEZ:AV0Z50040702
Institutional support: RVO:68081707
Keywords : Chloroplast DNA * Rumex acetosa * Sex chromosomes
Subject RIV: BO - Biophysics
Impact factor: 1.561, year: 2014

Chloroplast DNA (cpDNA) sequences are often found in plant nuclear genomes, but patterns of their chromosomal distribution are not fully understood. The distribution of cpDNA on the sex chromosomes can only be studied in dioecious plant species possessing heteromorphic sex chromosomes. We reconstructed the whole chloroplast genome of Rumex acetosa (sorrel, XY1Y2 system) from next generation sequencing data. We systematically mapped the chromosomal localization of various regions of cpDNA in R. acetosa and in Silene latifolia (white campion, XY system) using fluorescence in situ hybridization. We found that cpDNA was accumulated on the Y chromosomes of both studied species. In R. acetosa, the entire Y chromosome gathered all parts of cpDNA equally. On the contrary, in S. latifolia, the majority of the cpDNA, corresponding to the single copy regions, was localized in the centromere of the Y chromosome, while the inverted repeat region was present also in other loci.
Permanent Link: http://hdl.handle.net/11104/0230762