The isolation and characterization of lipopolysaccharides from Microcystis aeruginosa, a prominent toxic water bloom forming cyanobacteria

0424726 - BFÚ 2014 RIV GB eng J - Journal Article
Blahová, L. - Adamovský, O. - Kubala, Lukáš - Šindlerová, Lenka - Zounková, R. - Blaha, L.
The isolation and characterization of lipopolysaccharides from Microcystis aeruginosa, a prominent toxic water bloom forming cyanobacteria.
Toxicon. Roč. 76, DEC (2013), s. 187-196. ISSN 0041-0101. E-ISSN 1879-3150
Grant - others:GA ČR(CZ) GP13-27695P
Institutional support: RVO:68081707
Keywords : CYLINDROSPERMOPSIS-RACIBORSKII * ESCHERICHIA-COLI * DANIO-RERIO
Subject RIV: BO - Biophysics
Impact factor: 2.581, year: 2013

Massive toxic blooms of cyanobacteria represent a major threat to water supplies worldwide, yet serious gaps exist in understanding their complex toxic effects, including the role of lipopolysaccharides (LPS). The present comparative study focused on the levels and biological activities of LPS isolated from Microcystis aeruginosa, which is one of the most globally distributed toxic species. Using hot phenol extraction, LPS was isolated from 3 laboratory cultures and 11 natural water blooms. It formed 0.2-0.7% of the original dry biomass of the cyanobacteria, based on gravimetry. Additional analyses by commercial anti-LPS ELISA were correlated with gravimetry but showed concentrations that were about 7-times lower, which indicated either impurities in isolated LPS or the poor cross-reactivity of the antibodies used. LPS isolates from M. aeruginosa were potent pyrogens in the traditional Limulus amebocyte lysate (LAL)-test, but comparison with the PyroGene test demonstrated the limited selectivity of LAL with several interferences.
Permanent Link: http://hdl.handle.net/11104/0230761