Novel Aeruginosin-865 from Nostoc sp. as a Potent Anti-inflammatory Agent

0424616 - MBÚ 2014 RIV DE eng J - Journal Article
Kapuscik, Alexandra - Hrouzek, Pavel - Kuzma, Marek - Bártová, Simona - Novák, Petr - Jokela, J. - Pfluger, M. - Eger, A. - Hundsberger, H. - Kopecký, Jiří
Novel Aeruginosin-865 from Nostoc sp. as a Potent Anti-inflammatory Agent.
Chembiochem. Roč. 14, č. 17 (2013), s. 2329-2337. ISSN 1439-4227. E-ISSN 1439-7633
R&D Projects: GA MŠMT(CZ) LH11129; GA MŠMT(CZ) ED2.1.00/03.0110
Institutional support: RVO:61388971
Keywords : NMR spectroscopy * anti-inflammatory * cyanobacteria
Subject RIV: CE - Biochemistry
Impact factor: 3.060, year: 2013

Aeruginosin-865 (Aer-865), isolated from terrestrial cyanobacterium Nostoc sp. Lukesova 30/93, is the first aeruginosin-type peptide containing both a fatty acid and a carbohydrate moiety, and is the first aeruginosin to be found in the genus Nostoc. Mass spectrometry, chemical and spectroscopic analysis as well as one- and two-dimensional NMR and chiral HPLC analysis of Marfey derivatives were applied to determine the peptidic sequence: D-Hpla, D-Leu, 5-OH-Choi, Agma, with hexanoic and mannopyranosyl uronic acid moieties linked to Choi. We used an AlphaLISA assay to measure the levels of proinflammatory mediators IL-8 and ICAM-1 in hTNF-alpha-stimulated HLMVECs. Aer-865 showed significant reduction of both: with EC50 values of (3.51.5) mugmL(-1) ((4.01.7) muM) and (50.013.4) mugmL(-1) ((57.815.5) muM), respectively. Confocal laser scanning microscopy revealed that the anti-inflammatory effect of Aer-865 was directly associated with inhibition of NF-kappaB translocation to the nucleus. Moreover, Aer-865 did not show any cytotoxic effect
Permanent Link: http://hdl.handle.net/11104/0231317