Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens

0423951 - ÚEB 2014 RIV US eng J - Journal Article
Holá, Marcela - Kozák, Jaroslav - Vágnerová, Radka - Angelis, Karel
Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens.
BioMed Research International. -, ID 535049 (2013). ISSN 2314-6133. E-ISSN 2314-6141
R&D Projects: GA ČR GA13-06595S; GA MŠMT(CZ) LD13006
Institutional support: RVO:61389030 ; RVO:61388963
Keywords : DNA-DAMAGE * COMET ASSAY * ARABIDOPSIS-THALIANA
Subject RIV: EB - Genetics ; Molecular Biology

The moss Physcomitrella patens is unique for the high frequency of homologous recombination, haploid state, and filamentous growth during early stages of the vegetative growth, which makes it an excellent model plant to study DNA damage responses. We used single cell gel electrophoresis (comet) assay to determine kinetics of response to Bleomycin induced DNA oxidative damage and single and double strand breaks in wild type and mutant lig4 Physcomitrella lines. Moreover, APT gene when inactivated by induced mutations was used as selectable marker to ascertain mutational background at nucleotide level by sequencing of the APT locus. We show that extensive repair of DSBs occurs also in the absence of the functional LIG4, whereas repair of SSBs is seriously compromised. From analysis of induced mutations we conclude that their accumulation rather than remaining lesions in DNA and blocking progression through cell cycle is incompatible with normal plant growth and development and leads to sensitive phenotype.
Permanent Link: http://hdl.handle.net/11104/0229995