Extracellular Enzymes of the White-Rot Fungus Fomes fomentarius and Purification of 1,4-beta-Glucosidase

0423807 - MBÚ 2014 RIV US eng J - Journal Article
Větrovský, Tomáš - Baldrian, Petr - Gabriel, Jiří
Extracellular Enzymes of the White-Rot Fungus Fomes fomentarius and Purification of 1,4-beta-Glucosidase.
Applied Biochemistry and Biotechnology. Roč. 169, č. 1 (2013), s. 100-109. ISSN 0273-2289. E-ISSN 1559-0291
R&D Projects: GA MŠMT(CZ) LA10001; GA MŠMT OC08050
Institutional support: RVO:61388971
Keywords : Cellulose * 1,4-beta-glucosidase * Glycosyl hydrolase
Subject RIV: EE - Microbiology, Virology
Impact factor: 1.687, year: 2013

Production of the lignocellulose-degrading enzymes endo-1,4-beta-glucanase, 1,4-beta-glucosidase, cellobiohydrolase, endo-1,4-beta-xylanase, 1,4-beta-xylosidase, Mn peroxidase, and laccase was characterized in a common wood-rotting fungus Fomes fomentarius, a species able to efficiently decompose dead wood, and compared to the production in eight other fungal species. The main aim of this study was to characterize the 1,4-beta-glucosidase produced by F. fomentarius that was produced in high quantities in liquid stationary culture (25.9 U ml(-1)), at least threefold compared to other saprotrophic basidiomycetes, such as Rhodocollybia butyracea, Hypholoma fasciculare, Irpex lacteus, Fomitopsis pinicola, Pleurotus ostreatus, Piptoporus betulinus, and Gymnopus sp. (between 0.7 and 7.9 U ml(-1)). The 1,4-beta-glucosidase enzyme was purified to electrophoretic homogeneity by both anion-exchange and size-exclusion chromatography
Permanent Link: http://hdl.handle.net/11104/0229878